Reconstitution Guide

Reconstituting lyophilized peptides

Lyophilized (freeze-dried) peptides arrive as a solid powder and must be reconstituted with a suitable solvent before use in research. This guide covers the general laboratory procedure.

What you'll need

• Your lyophilized peptide vial
• Bacteriostatic or sterile water (the solvent)
• A sterile syringe
• Alcohol swabs

General procedure

1. Allow the vial to reach room temperature.
2. Swab both vial stoppers with alcohol.
3. Draw your chosen volume of solvent into the syringe.
4. Inject the solvent slowly down the inside wall of the vial — never directly onto the powder.
5. Let it dissolve on its own, or swirl gently. Do not shake.
6. Once fully clear, the peptide is reconstituted.

Use our peptide calculator to work out draw volumes for your target amount per draw.

This guide describes generic laboratory technique for research reagents only. It is not medical guidance.